X-gal; 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside

اطلاعات محصول:

Formula:                  C14H15BrClNO6

Molecular weight:      408.64 g/mol

CAS number:             7240-90-6

EINECS:                    230-640-8

Beilstein:                   1402009

P-Statement:              P261, P262

Synonyms:                 X-beta-D-galactoside

Abbreviations:            X-Gal; BClG

PubChem-ID:             49746937

MDLMFCD:                00005666

Handlingstore at -15°C, protect from light

اطلاعات فنی

دستورالعمل استفاده:

X-gal را توسط فیلتر استریل کرده و پس از استریل نمودن محیط کشت از محلوط ذخیره غلیظ آن به محیط بیافزایید.

:ویژگی ها

Solubility: ≥ 100 mM (40.9 mg/mL) in dimethyl sulfoxide ≥ 50 mM (20.4 mg/mL) in dimethyl formamide ≥ 6 mM (2.45 mg/mL) in ethanol

Recommended working concentration 0.1-0.3 mM (2-6 mL of 50 mM stock solution per L)

For best results also add 0.1-1 mM of the inducer isopropyl-beta-D-thiogalactopyranoside (IPTG, Biosynth product No. I-8000) to growth media Stability in growth media and aqueous solution: X-Gal was stable for ≥ 3 weeks at 37°C in nutrient agar and in phosphate buffer pH 7.3